Tarzan and giant spiders seem to go together, I feel. At least on screen. In the original ERB novels I'm not so sure, as I've only just started reading them. Maybe it's the cryptozoological stories of the J'ba Fo'fi, but deepest colonial Africa and giant arachnids seem a good fit. So much so I would be surprised if they weren't featured on a semi regular basis.
That said, extensive Googling has revealed only Desert Mystery. I would LOVE to see any more.
The Ron Ely tv show, which was the Tarzan of many people now between 35-45 (being slightly older, my Tarzan is Weissmuller) was surely too low budget, and low on special effects to have featured them? And in colour, which my memories aren't. But- we had a black and white tv until at least 1973, and I had one in my bedroom a lot later than that. And memories, eh? Who can trust them?
SBT